ʻO EndoFree Maxi Plasmid Kit

No ka hoʻomaʻemaʻe o ka endotoxin-free transfection grade plasmid DNA.

Hoʻokumu ʻia ka EndoFree Maxi Plasmid Kit ma ka ʻenehana membrane silica e huki ai i ka endotoxin-free plasmid DNA. Hāʻawi kēia kit i kahi kaʻina wehe endotoxin kūikawā e hana i kahi DNA plasmid kiʻekiʻe loa no nā noi i lalo. Hoʻonohonoho ʻia e hoʻokaʻawale i ka DNA plasmid o ka maʻemaʻe kiʻekiʻe loa mai 100-200 ml o ka moʻomeheu bacteria a hiki ke hāʻawi i ka 1.5 mg transfection grade plasmid DNA.

Pōpoki. Aʻole Ka hōʻiliʻili nui
4992194 10 preps

ʻIke kikoʻī

Laʻana Hoʻokolohua

Nā huahana huahana

Hiʻona

■ Loaʻa wikiwiki a kiʻekiʻe: 200 μg-1.5 mg plasmid DNA hua i 1 hola me ka hapa nui o ka super-coiled structure.
■ Maʻemaʻe kiʻekiʻe: Loaʻa ka DNA maemae kiʻekiʻe e ka ʻōnaehana buffer kū hoʻokahi a me Spin Column CP6.
■ Ka maikaʻi o ka hoʻololi ʻana: kūpono no nā hoʻokolohua transfection o ka nui o nā laina pūnaewele.
■ Ka laulā o nā noi: kūpono no ka palena ʻana o ka digestion enzyme, ka hoʻololi ʻana, ke kaʻina ʻana, ka microinjection, ka hoʻopaʻapaʻa ʻana i nā ʻenekini a me nā hoʻokolohua transfection.

Hāʻawi Plasmid

Plasmid Yield

Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)


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    Experimental Example Kiʻi kiʻekiʻe / haʻahaʻa kope plasmid
    ʻO ka plasmid pBR322 plopyid haʻahaʻa, i unuhi ʻia mai ka moʻomeheu bacteria 200 ml me ka hoʻohana ʻana iā EndoFree Maxi Plasmid Kit, ua elute ʻia i ka 1 ml Buffer TB, me ka hoʻonui o 0.6 μg / μl. ʻO ka pBS plasmid kope kiʻekiʻe, i unuhi ʻia mai ka moʻomeheu bacteria 100 ml e hoʻohana ana iā EndoFree Maxi Plasmid Kit, ua elute i 1 ml Buffer TB, me ka noʻonoʻo 1.2 μg / μl.
    MIV: TIANGEN DNA Marker IV
    pBR322: 2 μl, pBS: 2 μl
    Experimental Example Ua kiʻi ʻokoʻa ʻia ʻo PEGFP me EndoFree Maxi Plasmid Kit i laina laina 293T endotoxin-insensitive 293T a me nā pūnaewele Huh7 pūnaewele endotoxin-sensitive na TIANGEN TIANfect. ʻIke ʻia ka hōʻike o GFP i 24 mau hola ma hope o ka hoʻololi.
    Kākau i kāu leka ma aneʻi a hoʻouna iā mākou