■ Loaʻa wikiwiki a kiʻekiʻe: 200 μg-1.5 mg plasmid DNA hua i 1 hola me ka hapa nui o ka super-coiled structure.
■ Maʻemaʻe kiʻekiʻe: Loaʻa ka DNA maemae kiʻekiʻe e ka ʻōnaehana buffer kū hoʻokahi a me Spin Column CP6.
■ Ka maikaʻi o ka hoʻololi ʻana: kūpono no nā hoʻokolohua transfection o ka nui o nā laina pūnaewele.
■ Ka laulā o nā noi: kūpono no ka palena ʻana o ka digestion enzyme, ka hoʻololi ʻana, ke kaʻina ʻana, ka microinjection, ka hoʻopaʻapaʻa ʻana i nā ʻenekini a me nā hoʻokolohua transfection.
Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)
Kiʻi kiʻekiʻe / haʻahaʻa kope plasmid ʻO ka plasmid pBR322 plopyid haʻahaʻa, i unuhi ʻia mai ka moʻomeheu bacteria 200 ml me ka hoʻohana ʻana iā EndoFree Maxi Plasmid Kit, ua elute ʻia i ka 1 ml Buffer TB, me ka hoʻonui o 0.6 μg / μl. ʻO ka pBS plasmid kope kiʻekiʻe, i unuhi ʻia mai ka moʻomeheu bacteria 100 ml e hoʻohana ana iā EndoFree Maxi Plasmid Kit, ua elute i 1 ml Buffer TB, me ka noʻonoʻo 1.2 μg / μl. MIV: TIANGEN DNA Marker IV pBR322: 2 μl, pBS: 2 μl |
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Ua kiʻi ʻokoʻa ʻia ʻo PEGFP me EndoFree Maxi Plasmid Kit i laina laina 293T endotoxin-insensitive 293T a me nā pūnaewele Huh7 pūnaewele endotoxin-sensitive na TIANGEN TIANfect. ʻIke ʻia ka hōʻike o GFP i 24 mau hola ma hope o ka hoʻololi. |
Mai kona hoʻokumu ʻia ʻana, ke hoʻomohala nei kā mākou hale hana i nā huahana papa honua mua me ka hoʻopaʻa ʻana i ke ʻano
o ka maikaʻi ma mua. Ua loaʻa i kā mākou huahana ka maikaʻi maikaʻi loa i ka ʻoihana a me ka waiwaiabletrusty i waena o nā mea kūʻai aku a kahiko.