ʻO EndoFree Midi Plasmid Kit

No ka hoʻomaʻemaʻe wikiwiki o ka nui waena o endotoxin-free transfection grade plasmid DNA.

Hoʻokomo ka EndoFree Midi Plasmid Kit i kahi ʻenehana adsorption silikino membrane kū hoʻokahi i ka maikaʻi a hoʻopaʻa pono i ka DNA plasmid. Hiki i ka Buffer EBT kūikawā a me ka Filtration CS1 ke hoʻopau pono i nā impurities e like me endotoxin a me protein. ʻO ka hana hoʻoiho holoʻokoʻa he 1 wale nō hola, kahi maʻalahi a wikiwiki hoʻi.

Pōpoki. Aʻole Ka hōʻiliʻili nui
4992853 10 preps

ʻIke kikoʻī

Kahua hana

Laʻana Hoʻokolohua

Nā huahana huahana

Hiʻona

■ Kūpono no ka lawe ʻana mai i nā plasmids mai ka medium medium moʻomeheu bacteria 50-100 ml.
■ Loaʻa i ka plasmid i hoʻomaʻemaʻe ʻia ka endotoxin haʻahaʻa loa a hiki ke hoʻokō i nā koina o ka transfection kiʻekiʻe.
■ Maʻalahi e hana me kahi hana ʻokoʻa a wikiwiki endotoxin.

Nā noi noi

Hiki ke hoʻopili ʻia ka DNA plasmid hoʻomaʻemaʻe i nā hoʻokolohua biology molekiola a me nā hoʻokolohua biology pūnaewele e like me ka palena endonuclease digestion, ke kaʻina ʻana, ka hoʻololi ʻana, ka hoʻololi ʻana, microinjection, etc.

Hāʻawi Plasmid

Yield

Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)


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    Workflow

    Workflow Hoʻomaʻemaʻe ʻia ka Plasmid pEGFP-C1 e ka EndoFree Midi Plasmid Kit mai 50 ml i ka pō i waena o ka bacteria bacteria. Ua ana ʻia ka lehulehu plasmid e Nanodrop a ua helu ʻia ka hua. Hōʻike ka hopena i ka hua plasmid na EndoFree Mini Plasmid Kit ʻoi aku ka kiʻekiʻe ma mua o ka huahana e pili ana mai ka mea hoʻolako A.
    Workflow ʻO ka plasmid pEGFP-C1 i unuhi ʻia e EndoFree Midi Plasmid Kit ua hoʻololi ʻia i 80% confluence 293T pūnaewele e TIANGEN TIANfect. ʻIke ʻia ka hōʻike o GFP 24-36 h ma hope o ka hoʻololi. Hōʻike ka hopena i ka hopena transfection 80% -90%.
    Kākau i kāu leka ma aneʻi a hoʻouna iā mākou