■ Loaʻa wikiwiki a me nā kiʻekiʻe: 5-70 μg hiki ke lawe ʻia mai DNA plasmid ma kahi o 1 hola.
■ ʻO ka maʻemaʻe kiʻekiʻe: ʻO ka ʻōnaehana hoʻopau endotoxin kūikawā i hui pū ʻia me Spin Column CP4 hiki kikoʻī adsorb nucleic acid a hemo i ka endotoxin i ka pae liʻiliʻi.
■ Ka laulā o nā noi: kūpono no nā hoʻokolohua maʻamau e like me ka restriction endonuclease digestion, PCR, ke kaʻina ʻana, ka ligation, ka hoʻololi ʻana, a me nā hoʻokolohua kiʻekiʻe loa e like me ka gen therapy, cell microinjection, genencing silence, transcription, etc.
Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)
Kūpono no ka unuhi ʻana o nā plasmids kope kiʻekiʻe / haʻahaʻa Kiʻi 1. Ka unuhi ʻana o ka plasmid DNA mai nā ʻano like ʻole o ka moʻomeheu bacteria i hoʻohana ʻia ʻo EndoFree Mini Plasmid Kit II. Volume Elution: 200 μl; Ke hoʻouka nei i ka leo: 3 μl haʻahaʻa-kope plasmid pBR322, 2 μl kope kiʻekiʻe plasmid pBS. Ua alakaʻi ʻia ka electrophoresis ma 6 V / cm no 30 min ma 1% agarose. ʻO ka transfection pūnaewele e ka plasmid hoʻomaʻemaʻe |
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Hōʻike 2 Ua hoʻololi ʻia ka plasmid pEGFP e EndoFree Mini Plasmid Kit II i nā pūnaewele 293T. ʻIke ʻia ka hōʻike o GFP ma 48 h post-transfection |
Mai kona hoʻokumu ʻia ʻana, ke hoʻomohala nei kā mākou hale hana i nā huahana papa honua mua me ka hoʻopaʻa ʻana i ke ʻano
o ka maikaʻi ma mua. Ua loaʻa i kā mākou huahana ka maikaʻi maikaʻi loa i ka ʻoihana a me ka waiwaiabletrusty i waena o nā mea kūʻai aku a kahiko.