EndoFree Mini Plasmid Kit II

No ka hoʻomaʻemaʻe o ka endotoxin-free transfection grade plasmid DNA.

Hoʻokomo ka EndoFree Mini Plasmid Kit II i kahi ʻenehana adsorption silikino membrane kū hoʻokahi i ka maikaʻi a hoʻopaʻa pono i ka DNA plasmid. Hiki i ka ʻōnaehana endotoxin-hemo kūikawā ke hoʻopau pono i nā impurities e like me endotoxin a me nā protein. ʻO ke kaʻina hana unuhi holoʻokoʻa he 1 wale nō hola, kahi maʻalahi a wikiwiki hoʻi. Pili ka hua hoʻoiho plasmid i ka helu kope plasmid, ʻano bacteria a me nā ʻano moʻomeheu. Hiki i ka 70 μg o ka plasmid DNA ke lawe ʻia mai 5-15 ml o ka pōʻaiapili moʻomeheu moʻomeheu i ka pō.

Pōpoki. Aʻole Ka hōʻiliʻili nui
4992422 50 preps

ʻIke kikoʻī

Laʻana Hoʻokolohua

Nā huahana huahana

Hiʻona

■ Loaʻa wikiwiki a me nā kiʻekiʻe: 5-70 μg hiki ke lawe ʻia mai DNA plasmid ma kahi o 1 hola.
■ ʻO ka maʻemaʻe kiʻekiʻe: ʻO ka ʻōnaehana hoʻopau endotoxin kūikawā i hui pū ʻia me Spin Column CP4 hiki kikoʻī adsorb nucleic acid a hemo i ka endotoxin i ka pae liʻiliʻi.
■ Ka laulā o nā noi: kūpono no nā hoʻokolohua maʻamau e like me ka restriction endonuclease digestion, PCR, ke kaʻina ʻana, ka ligation, ka hoʻololi ʻana, a me nā hoʻokolohua kiʻekiʻe loa e like me ka gen therapy, cell microinjection, genencing silence, transcription, etc.

Hāʻawi Plasmid

Plasmid Yield

Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)


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    Experimental Example Kūpono no ka unuhi ʻana o nā plasmids kope kiʻekiʻe / haʻahaʻa
    Kiʻi 1. Ka unuhi ʻana o ka plasmid DNA mai nā ʻano like ʻole o ka moʻomeheu bacteria i hoʻohana ʻia ʻo EndoFree Mini Plasmid Kit II.
    Volume Elution: 200 μl; Ke hoʻouka nei i ka leo: 3 μl haʻahaʻa-kope plasmid pBR322, 2 μl kope kiʻekiʻe plasmid pBS.
    Ua alakaʻi ʻia ka electrophoresis ma 6 V / cm no 30 min ma 1% agarose.
    ʻO ka transfection pūnaewele e ka plasmid hoʻomaʻemaʻe
    Experimental Example Hōʻike 2 Ua hoʻololi ʻia ka plasmid pEGFP e EndoFree Mini Plasmid Kit II i nā pūnaewele 293T. ʻIke ʻia ka hōʻike o GFP ma 48 h post-transfection
    Kākau i kāu leka ma aneʻi a hoʻouna iā mākou