I Kēkēmapa 2019, ua hoʻomaka ʻia kahi hanana pneumonia o nā kumu ʻike ʻole ʻia mai Wuhan, Hubei Province, a hoʻolaha koke ʻia i ka hapa nui o nā panalāʻau a me nā kūlanakauhale ma Kina, a me nā ʻāina ʻē he nui i Ianuali 2020. E like me 22:00 pm ma Ianuali 27, 28 mau hihia i hōʻoia a 5794 i manaʻo ʻia he mau hihia 2019-nCov i hōʻike ʻia ma Kina. ʻO kaua kuhi ʻia ke kumu maʻi o ka maʻi ma ke ʻano he Rhinolophus, a ʻo ka helu make ma kēia manawa he 2.9%.
Ma Ianuali 12, 2020, ua hoʻolaha ka World Health Organization (WHO) ua hoʻokumu ʻia ka maʻi maʻi pneumonia e kekahi ʻano coronavirus (2019-nCov). ʻO Coronavirus kekahi ʻano o nā mea maʻi i pālahalaha aʻe ma waena o nā holoholona. ʻO ka maʻi virus nucleic acid he RNA kū hoʻokahi. I ka manawa like, ua hoʻokuʻu ʻia ʻo WHO i ka ʻike kaʻina kōmike nukelika o nCov i kaʻanalike ʻia e nā poʻe ʻepekema Kina, nāna i hāʻawi i ke kumu o ka huli ʻana o ka moleola no ka loaʻa ʻana o ka maʻi a ua hiki ke loaʻa koke a me ka hoʻomaopopo ʻana i nā pathogens.
Hōʻike ʻo WHO i ka poʻe i loaʻa i nā maʻi hanu koʻikoʻi e like me ka hano, kuni, maʻi maʻi hanu a ua hele i Wuhan i loko o 14 mau lā a i ʻole i ʻike ʻia e hoʻopili me nā mea maʻi ʻē aʻe e pono e hoʻāʻo. Ma ka 17 Ianuali 2020, ua hoʻokuʻu ʻo WHO i ka "Laboratory Testing for 2019 novel coronavirus (2019-nCoV) i nā hihia kanaka i manaʻo ʻia, alakaʻi kūwaho, 17 Ianuali 2020". Kuhikuhi ke alakaʻi i nā hiʻohiʻona e ʻohiʻohi ʻia mai nā mea maʻi hōʻailona me nā hiʻohiʻona hanu (nasopharynx a me nā swabs oropharyngeal, sputum, bronchoalveolar lavage, a pēlā aku) a me nā hiʻohiʻona serum, penei:
* No ka lawe ʻana i nā laʻana no ka ʻike viral, e hoʻohana iā VTM (medium transport viral) i loko o nā mea hoʻopili antifungal a me nā lāʻau antibiotic. No ka moʻomeheu bacterial a fungal paha, lawe i kahi maloʻo a i loko paha o kahi wai liʻiliʻi liʻiliʻi o ka wai sterile. Hōʻalo i ke anuanu mau a me ka hoʻoheheʻe ʻana o nā specimens.
Ma waho o nā mea hōʻiliʻili kikoʻī i hōʻike ʻia i ka papaʻaina e hōʻoia hoʻi i ka loaʻa o nā pono a me nā pono ʻē aʻe. nā lepili a me nā māka paʻa, PPE, nā pono hana no ka hoʻohaumia ʻana o nā ʻilikai.
No ka ʻike ʻana i nā mea hoʻomaʻi, hiki ke hoʻohana ʻia nā laikini kuhi e ʻike i ka maʻi ma o ka hana immunological, ʻoiai e koi ʻia ʻo RTqPCR e ʻike i ka waikona nCov nucleic no ka ʻike wikiwiki a me ka ʻoi. E like me ka mea hoʻomaʻi
ʻike ʻia ke kaʻina, hiki ke ʻike koke ʻia ka ʻike o ka acidic acidic nucleic i loko o ka specimen e ka wale nō
ke koho ʻana i nā reagents kūpono a me nā primers like
(ʻIke ʻike diagnostic o Wuhan coronavirus 2019 e ka manawa maoli RTPCR, Pūnaewele a me ka loiloi mua e like me Ian 13, 2020) Ma ke ʻano he alakaʻi i ka māina o ka wehe ʻana o ka waikawa nucleic a me ka ʻike ʻana ma Kina, TIANGEN BIOTECH (BEIJING) CO., LTD. ua hāʻawi i ka maʻi wawae lima-wāwae-waha a me nā mea hoʻomaʻi ʻike virus ʻo influenza A (H1N1) no 10 miliona mau kānaka. I ka makahiki 2019, ua hoʻohana ʻia ʻo TIANGEN's virus nucleic acid extraction and detection reagents i 30 miliona mau ʻāpana puaʻa puaʻa ʻApelika, e hāʻawi ana i nā makana maikaʻi loa i ka ʻike a me ka pale ʻana i ke kuni puaʻa kūpaka ʻApelika ma Kina. Hāʻawi wale ʻo TIANGEN i ka wikiwiki a me ka pololei o ka wehe ʻana i nā maʻi a me nā mea loaʻa, akā hāʻawi pū kekahi i nā mea wili a me ka pono o nā mea wili a me nā mea lawe ʻino nucleic acid, ka mea i piha i nā kahe no ka unuhi ʻana o ka maʻi a me ka ʻike.
Pāʻoihana Nucleic Acid Extraction Solution
ʻO TIANGEN Automated Nucleic Acid Extractors he pae maʻalahi no ka lawe ʻana o ka waikawa nukelika me ka hoʻohana ʻana i ka metala bead. ʻO ka noi ʻana o kēia mau paepae ʻaʻole ia e hoʻoliʻiliʻi nui i ka hana o ka nānā ʻana a me nā keʻena quarantine, akā hoʻoliʻiliʻi hoʻi i nā hewa hana o ka unuhi lima ʻana, a hōʻoia i ke kūlike a me ke kūpaʻa o ka maikaʻi o nā acidic nucleic i lawe ʻia.
ʻO TIANGEN Automated Nucleic Acid Extractor he mau ʻano hana like ʻole (e like me 16, 24, 32, 48, 96 auwai), a hiki ke hoʻohana ʻia nā mea hoʻopili like no ka unuhi ʻana o ka waikawa nukelika mai nā ʻano laʻana like ʻole. Hāʻawi pū ʻo TIANGEN i ka hoʻomohala reagent a me nā lawelawe hoʻohuihui i nā mea hana e like me nā koi hoʻokolohua like ʻole.
ʻO TGrinder H24 Tissue Homogenizer
● Hoʻohana ākea ʻia no ka wili ʻana a me ka homogenizing ʻana i nā mea like ʻole a me nā hāpana feces
● Me ka wili homogenization ikaika 2-5 mau manawa o nā mea hana kuʻuna
● Ke wili ʻana a me ka homogenization ʻana, ka pale ʻana i ka haumia o ke keʻa
● Mea pale pilikino e pale i ka palekana o nā limahana o ke keʻena hoʻokolohua
ʻO TGuide S32 Automated Nucleic Acid Extractor
● Hōʻailona laʻana: 1-32 mau laʻana
● Ka leo hana: 20-1000 μl
● ʻAno laʻana: Koko, nā hunaola, nā ʻaʻa, nā feces, nā mea hoʻomaʻi a me nā hāpana ʻē aʻe
● Ka manawa hana: A i 8 mau min e loaʻa ai ka acidic nucleic acid
● ʻAno kaohi: Ke ʻano kaohi pālua o ka Windows Pad a me ka pihi pale
● Hoʻomohala paepae: Wehe i ke anuu, manuahi e hoʻokūkū i nā reagents
● TGuide S32 Mākēneki Viral DNA / RNA Kit (DP604)
● Ke noi ākea: Hiki ke hoʻomaʻemaʻe ʻia ka maʻi virus DNA / RNA kiʻekiʻe loa mai ka serum, ka plasma, ka hāpana swab, ka hopena lapaʻau a me nā hopena mālama virus like ʻole.
● maʻalahi a me ka pono: Hoʻohālikelike pono ʻia kēia huahana me TGuide S32 Automated Nucleic Acid Extractor, hiki ke huki i ka virus DNA / RNA me nā hua kiʻekiʻe, ke kiʻekiʻe kiʻekiʻe, kūpaʻa a me ka maikaʻi kūpaʻa.
● Nā noi i lalo: kūpono ka acidic acid i hoʻomaʻemaʻe ʻia no nā hoʻokolohua kahe o ka virus PCR a me ka PCR manawa maoli.
Pāʻoihana Nucleic Acid Extraction Solution
Ma ke ʻano he alakaʻi i ke kahua o ka wehe ʻana a me ka ʻike ʻana o ka waikawa nukelika, loaʻa iā TIANGEN ka pūʻulu ʻoi loa o nā huahana hoʻoiho waikawa nucleic ma Kina, kahi kūpono no ka ʻike coronavirus mai nā ʻano mea like ʻole: ke koko, serum / plasma, tissue, swab, virus , etc.
ʻO TIANamp Virus DNA / RNA Kit (DP315)
● Ka maikaʻi kiʻekiʻe: Hiki ke kiʻi ʻia ka maʻi virus kiʻekiʻe a me RNA e ka hoʻomaʻemaʻe wikiwiki me ka hoʻihoʻi kiʻekiʻe.
● ʻO ka maʻemaʻe kiʻekiʻe: Lawe loa i nā mea haumia a me nā mea hoʻohiehie no ka noi i lalo.
● Ka palekana kiʻekiʻe: ʻAʻole pono ka unuhi ʻana o ka meaola a i ʻole ka hoʻoue ʻana o ka etanol.
Hālāwai RNA Virus Detection
1. TEasy Pipetting System ʻōnaehana
● Kūpono kiʻekiʻe: Hiki i ka palaka hōʻoluʻolu ke mālama i ka mahana o ka laʻana reagent ma lalo o 7 ℃ no ka mea he 60 mau min. Hoʻopili ʻia kēlā me kēia APM e like me nā kūlana o ka honua, ʻoi aku ka kiʻekiʻe o ke kikoʻī ma mua o ka pipetting manual.
● Hana maʻalahi: Ka liʻiliʻi liʻiliʻi. Kaona māmā. ʻAʻole pono kahi mea hana no nā mea pani hou. ʻO ke kaʻina hana hoʻomākaukau PCR / qPCR i kūkulu ʻia. ʻO ka hana lima maʻalahi o ka hoʻomākaukau hoʻonā PCR.
● Ke noi ākea: Hiki ke hoʻohana ʻia no ka pipetting pā 96/384-maikaʻi, PCR, qPCR, ʻike ʻana i nā ʻāpana a me nā hoʻokolohua kiʻekiʻe kiʻekiʻe.
FastKing Kekahi ʻAnuʻu RT-qPCR Kit (ʻIke) (FP314)
ʻO ka FastKing One Step RT-qPCR Kit (Probe) (FP314) i hoʻomohala ʻia e TIANGEN kahi kit e ʻike pono i ka fluorescence ʻike nui i hoʻokumu ʻia ma ke ʻano o ka hana probe, kahi i hoʻomākaukau kūikawā ʻia no ka ʻike ʻana i nā genes trace i nā laʻana like ʻole. ʻO ka KingRTase i loko o ka pahu he molekole hou i hoʻololi hou ʻia i transcriptase, kahi i ʻoi aku ka ikaika o ka pilina RNA a me ke kūpaʻa pumehana, me ka maikaʻi o ka transcription hope a me ka hiki ke hoʻonui ʻia o nā anakuhi RNA. Hoʻohana ʻia kahi hoʻomaka wela Taq DNA polymerase e hāʻawi i ka hopena PCR i ka hoʻonui ʻana i ka pono a me ka kikoʻī. Hoʻohui ʻia, maʻalahi ka pahu i nā ʻāpana i ka nui loa ma ka hoʻohuihui ʻana i ka Taq a me ka MLV enzyme i loko o ka hoʻohui enzyme, a me ka buffer ion pre-mixing, dNTPs, PCR stabilizer a me ka enhancer i MasterMix, i hiki ai i nā pae hoʻohuihui maha.
simplified.
● Kūlana kiʻekiʻe: ʻO ka transcriptase hoʻohuli maikaʻi a me ka DNA polymerase e hōʻoia i ka hopena o ke ʻano kiʻekiʻe
● Hoʻohuli maikaʻi: Hiki i ka polymerase ke heluhelu ma o ka template RNA me nā ʻike GC kiʻekiʻe a me nā hanana lua paʻakikī
● Nā noi ākea: Ke hoʻohana kiʻekiʻe i nā template RNA me nā haumia mai nā ʻano ʻokoʻa
● ʻIke kiʻekiʻe: hiki ke ʻike pololei ʻia nā ʻōkuhi ma lalo o 1 ng, ʻoi aku hoʻi no nā anakuhi haʻahaʻa haʻahaʻa
Laʻana no ka ʻike ʻana o RNA Virus
ʻO ka acidic nucleic o H5 avian influenza i lawe ʻia e TGuide S32 Magnetic Viral DNA / RNA Kit (DP604). ʻO ka FastKing One Step RT-qPCR Kit (Probe) (FP314) i hoʻohana ʻia no ka ʻike RT-qPCR e hoʻohana ana i nā H5 avian influenza primers a me nā probes.
Ua hoʻohana ʻia ʻo ABi7500Fast no ka ʻike RT-qPCR. Nā hualoaʻa o ka H5 avian influenza virus antigen (10-2, 10-3, 10-4, 10-5, 10-6and 10-7 dilution) mai ka 200 μl mau laʻana e hōʻike nei i nā hua o ka huʻihuʻi nucleic acid kiʻekiʻe, a hiki ke hālāwai me ka mea e hiki mai ana. pono o ka huli hou ʻana, PCR, RT-PCR, PCR manawa maoli, a pēlā aku. Hāʻawi ka PCR manawa maoli i nā hopena me ke kiʻekiʻena kiʻekiʻe, ka hana hou ʻana a me ka linearity dilution gradient maikaʻi. Hiki ke ʻike pololei ʻia nā hoʻoliʻiliʻi ʻokoʻa o nā laʻana o nā nukelika viral.
Ka manawa o ka manawa: Apr-11-2021
