ʻO RNALock Reagent

No ka mālama ʻana i nā hāpana koko hou āpau no ka ʻekekeke acidic nucleic.

ʻO ka RNALock Reagent kahi ʻano wai, he mea mālama mālama koko ʻino ʻole. Hoʻopaʻa koke ia iā RNA i ke koko hou. Hiki ke mālama ʻia nā hāpana koko kanaka ma loko o ka reagent ma 2-8 ℃ no 5 mau lā, a i ʻole -20 ℃ no ʻekolu mau mahina. Hiki ke mālama ʻia nā hāpana koko mamala i loaʻa ka reagent ma 15-25 ℃ no 2 mau lā, 2-8 ℃ no 7 mau lā, a i ʻole -20 ℃ no ʻeono mau mahina. Ma lalo o kēia mau kūlana, ʻaʻole hōʻike RNA i ka hoʻohaʻahaʻa nui. Hiki i ka RNAprep Pure Kit Kit me kahi protocol i hoʻomākaukau ʻia ke hōʻoia i ka hemo wikiwiki ʻana o ka nui o ka RNA mai ke koko i mālama ʻia i ka RNALock Reagent.

Pōpoki. Aʻole	Ka hōʻiliʻili nui
4992731	100 ml

E hoʻouna leka uila iā mākou

ʻIke kikoʻī

Kahua hana

Laʻana Hoʻokolohua

Nā huahana huahana

Hiʻona

■ Ka palekana kūpono: pale iā RNA i ke koko hou mai ka hoʻohaʻahaʻa.
■ kūpono: lawe ka hana i 2 mau ʻanuʻu a kūpono no ka mālama ʻana i ka nui o nā laʻana koko.
■ Hoʻohālikelike kūpono: Kūlike me kā TIANGEN nona ponoʻī Silicon Matrix Membrane Purification Kit e huki ai i ke koko RNA a i ʻole DNA e hōʻoia i ka hua a me ka maʻemaʻe.

Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)

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Experimental Example

Pono: 100 μl koko iole hou
Nā hana: ʻO ka huina RNA o ke koko ʻiole hou (mālama ʻia ma RNALock Reagent) i mālama ʻia ma 15-25 ℃, 4-8 ℃, -20 / -70 ℃ i hoʻokaʻawale ʻia me ka hoʻohana ʻana i ka RNAprep Pure Blood Kit (4992238) ma hope o ka RNALock protocol .
Nā hopena: E ʻoluʻolu e ʻike i ke kiʻi ʻelala agarose gel electrophoresis ma luna. 4-6 μl o 50 μl eluates i hoʻouka ʻia i kēlā me kēia laina.
M: TIANGEN DNA Marker III;
Lane 1-2 (kaohi maikaʻi): Hoʻomaʻemaʻe ʻia ʻo RNA mai ke koko hou;
Lane 3-4: Hoʻomaʻemaʻe ʻia ʻo RNA mai nā hāpana koko i mālama ʻia ma 15-25 ℃ no 2 mau lā.
Lane 5-6: Hoʻomaʻemaʻe ʻia ka RNA mai nā hāpana koko i mālama ʻia ma 4-8 ℃ no hoʻokahi pule.
Lane 7-8: Hoʻomaʻemaʻe ʻia ka RNA mai nā hāpana koko i mālama ʻia ma -20 ℃ a i ʻole -70 ℃ no ka hapalua makahiki.
Lawe ʻia ka electrophoresis ma 6 V / cm no 30 min ma ka agarose 1%.

Kākau i kāu leka ma aneʻi a hoʻouna iā mākou