STIANqucik N96 Pūnaewele Hoʻomaʻemaʻe

Hoʻomaʻemaʻe kiʻekiʻe-kiʻekiʻe o nā ʻāpana 100 bp-10 kb DNA.

Hoʻohui pū ka pahu i nā ʻāpana DNA i nā hopena hopena e like me ka digestion o ka enzyme, PCR, ke kaʻina ma o ka N96 Plate CB2. Hiki iā ia ke hoʻoneʻe i nā impurities e like me nā protein, nā mea hoʻohui ʻokanika, nā iona paʻakai inorganic, nā oligonucleotide primers, a me nā mea ʻē aʻe i kūpono i ka DNA hoʻomaʻemaʻe no nā hoʻokolohua biology molekiola e like me ka digestion enzyme digestion, sequencing, library screening, ligation and transformation.

Pōpoki. Aʻole Ka hōʻiliʻili nui
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ʻIke kikoʻī

Kahua hana

Laʻana Hoʻokolohua

Nā huahana huahana

Hiʻona

■ Hiki ke piʻi aʻe ka helu o 100 bp-10 kb DNA ʻāpana ma mua o 80%.
■ ʻO ka nui o nā DNA adsorbed no kēlā me kēia luawai ʻo 5 µg.
■ Ka maʻemaʻe kiʻekiʻe: hiki ke hoʻohana pololei ʻia ka DNA huikala ʻia i nā hoʻokolohua kahawai.

 

Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)


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    Workflow

    900 hoʻomaʻemaʻe bp, 3500 bp a me 4500 bp i hoʻomaʻemaʻe ʻia me ka hoʻohana ʻana i ka TIANquick N96 Purification Kit.
    3 μl o 50 μl eluant i hoʻouka ʻia i ka gel agarose 1%, a ua hana ʻia ka electrophoresis ma 6 V / cm no 20 min.
    1, 2 a me 3: hoʻomaʻemaʻe ka DNA me ka hoʻohana ʻana iā TIANGEN TIANquick N96 Purse Kit;
    4: Hoʻomaʻemaʻe ʻo DNA i ka hoʻohana ʻana i ka huahana kūpono mai ka mea hoʻolako A.

    Experimental Example:

    Kākau i kāu leka ma aneʻi a hoʻouna iā mākou

    Nā huahana huahana