ʻO TIANamp FFPE DNA Kit
Hiʻona
■ Ka maʻemaʻe kiʻekiʻe: Hōʻalo ka membrane Silica i ka hapa nui o nā impurities.
■ Hoʻohana ākea: kūpono no ka ʻāpana paraffin, poloka paraffin, a me nā laʻana i hoʻopili ʻia formalin.
■ Hana wikiwiki: hiki ke kiʻi ʻia ʻo DNA ma loko o 1 hola.
Hoakaka
ʻAno: Hoʻokumu ke kolamu wili
Laʻana: Paukū paraffin, palaka paraffin, a me nā laʻana i hoʻopili ʻia formalin
Pahuhopu: Genomic DNA
Hoʻomaka ka leo: 5-8 ʻāpana o nā ʻāpana paraffin a i ʻole 30 mau kiko i loko o paraffin a i ʻole formalin
Ka manawa hana: ~ 1 hola
Nā noi i lalo: PCR, SNP genotype analysis, STR analysis, pharmacogenomics analysis, NGS hale waihona puke, etc.
Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)
ʻO ka genomic DNA i unuhi ʻia e TIANamp FFPE DNA Kit a me ka huahana pili mai ka mea hoʻolako A ua hoʻonui ʻia i 190 bp, 330 bp, 500 bp, 650 bp a me 1300 bp mau ʻāpana. Hōʻike ka hopena ʻo DNA i kāʻili ʻia e TIANamp FFPE DNA Kit he ʻoi aku ka kiʻekiʻe ma luna, ʻoi aku ka maʻemaʻe kiʻekiʻe no nā ʻāpana nui.
Ke hoʻomaka nei i nā mea: Nā laʻi ʻiole FFPE ʻiole (5 ʻāpana, 10 μm / ʻāpana)
Ke Kaʻina Hana: E hāhai i nā ʻōkuhi o kēlā me kēia huahana.
Ke hoʻouka nei i ka gel: ua hoʻonui ʻia ʻo gDNA e ka buffer elution 50 μl a ua hoʻonui ʻia i 190 bp, 330 bp 500 bp, 650 bp a me 1300 bp. 5 μl o 20 μl huahana PCR i hoʻouka ʻia i kēlā me kēia ala ma ka 1% agarose gel. Lawe ʻia ka electrophoresis ma 6 V / cm no 20 min.
M: TIANGEN Marker D2000;
T: TIANamp FFPE DNA kini;
A: Huahana kūpono mai ka mea hoʻolako A.
A-1 Haʻahaʻaha haʻahaʻa o nā hunaola a i ʻole virus i ka hōʻailona hoʻomaka - E hoʻonui i ka hoʻonui ʻia o nā hunaola a i ʻole nā virus.
A-2 lawa ʻole ka lisis o nā laʻana - ʻAʻole i huikau hui ʻia nā laʻana me ka buffer lysis. Manaʻo ʻia e kāwili pono ʻia e ka pulse-vortexing no 1-2 mau manawa. —No ka lawa ʻole o ka lysis pūnaewele i hoʻoliʻiliʻi ʻia e ka hana i hoʻoliʻiliʻi o proteinase K. ʻLelo ʻia e ʻokiʻoki i ke kiko i nā ʻāpana liʻiliʻi a hoʻolōʻihi i ka manawa ʻauʻau e lawe i nā koena āpau i ka lysate.
A-3 lawa ʻole ka adsorption DNA. —No ka ethanol a i ʻole ka pākēneka haʻahaʻa ma kahi o 100% ethanol i hoʻohui ʻia ma mua o ka hoʻoili ʻia ʻana o ka lysate i ke kolamu wili.
A-4 ʻOi aku ka waiwai o ka pH o ka buffer elution. — Hoʻoponopono i ka pH ma waena o 8.0-8.3.
Ethanol koena i ka eluent.
— Aia ke koena holoi pā pale PW ma nā eluent. Hiki ke hemo ʻia ka ethanol e ka centrifuging ʻana i ke kolamu wili no 3-5 min, a laila kau i ke ana wela a i ʻole 50 ℃ incubator no 1-2 min.
A-1 ʻAʻole hou ka laʻana. —E unuhi i kahi hāpana DNA kūpono e like me ke kaohi e hoʻoholo inā ua hoʻohaʻahaʻa ʻia ka DNA.
A-2 lapaʻau kūpono ʻole. —Noho ʻia e ka wili nui ʻana o ka nitrogen wai, hoʻāʻo hou, a i ʻole ka nui o nā hāpana.
Pono e hoʻololi i nā pretreatment no nā laʻana like ʻole. No nā mea kanu mea kanu, e wili pono i ka nitrogen wai. No nā laʻana holoholona, homogenate a wili maikaʻi paha i ka nitrogen wai. No nā laʻana me nā paia paʻa e paʻakikī e haki, e like me G + bacteria a me ka hū, ʻōlelo ʻia e hoʻohana i ka lysozyme, lyticase a i ʻole nā ʻano hana mechanical e haki i nā paia o ka pūnaewele.
4992201/4992202 Plant Genomic DNA Kit apono i ke kolamu hana-e koi ana i ka chloroform no ka unuhi. Kūpono ia no nā mea kanu mea kanu like ʻole, a me ka pauka maloʻo maloʻo. ʻO ka Hi-DNAsecure Plant Kit kahi kolamu hoʻi, akā ʻaʻohe pono o ka phenol / chloroform extraction, e palekana ana a ʻaʻohe mea ʻawahia. Kūpono ia no nā mea kanu me nā polysaccharides kiʻekiʻe a me nā ʻike polyphenol. 4992709/4992710 DNAquick Plant System apono i kahi hana wai-pili. ʻAʻole pono ʻo Phenol / chloroform extraction pū kekahi. He maʻalahi a wikiwiki hoʻi ke kaʻina hana hoʻomaʻemaʻe me ka palena ʻole no nā laʻana o ka laʻana, no laila hiki i nā mea hoʻohana ke hoʻomaʻalahi i ka nui e like me nā koi hoʻokolohua. Hiki ke kiʻi ʻia ka nui o nā ʻāpana gDNA me nā hua kiʻekiʻe.
Ua lawe ʻia ka genomic DNA mai nā puke like ʻole o nā laʻana koko kanaka āpau e TIANamp Blood DNA Kit. Penei nā hopena. Ua helu ʻia nā hopena ma ke ʻano he kūmole wale nō, nā hopena o ka unuhi maoli ʻana ma muli o nā kūlana o nā laʻana.
Hiki ke hoʻokō ʻia ke kāʻili ʻana o ka DNA kūpē ʻana i ke koko me ka hoʻohana ʻana i nā reagents i hāʻawi ʻia i loko o kēia mau pahu ʻelua ma o ka hoʻololi ʻana i ka protocol i ke kuhikuhi kikoʻī no ka wehe ʻana o ke koko koko. Hiki ke hoʻopuka i ke kope palupalu o ke kōkō DNA kāʻili i ke kaʻina koi ma ke noi ʻana.
Wehe i ka hāpana hou me 1 ml PBS, saline maʻamau a i ʻole buffer TE. Hoʻopiha piha i ka hāpana e kahi homogenizer a hōʻiliʻili i ka piʻi i lalo o kahi o ka pahu e centrifuging. E hoʻolei i ka mea kupanaha, a resuspend i ka piʻi ʻana me 200 μl buffer GA. Hiki ke hana i ka hoʻomaʻemaʻe DNA aʻe e like me ke aʻo.
No ka hoʻomaʻemaʻe o gDNA i ka plasma, serum a me nā laʻana wai o ke kino, paipai ʻia ʻo TIANamp Micro DNA Kit. No ka hoʻomaʻemaʻe ʻana i ka maʻi gDNA mai nā mea serum / plasma, makemake ʻia ʻo TIANamp Virus DNA / RNA Kit. No ka hoʻomaʻemaʻe ʻana i ka gDNA bacteria mai nā serum a me nā hāpana plasma, paipai ʻia ʻo TIANamp Bacteria DNA Kit (pono e hoʻokomo ʻia ka lysozyme no ka maʻi maikaʻi). No nā laʻana kuha, paipai ʻia ʻo Hi-Swab DNA Kit a me TIANamp Bacteria DNA Kit.
Kākoʻo ʻia ʻo DNAsecure Plant Kit a i ʻole DNAquick Plant System no ka lawe ʻana i ka genome fungal. No ka hemo genome hū, koi ʻia ʻo TIANamp Yeast DNA Kit (e hoʻomākaukau pono ʻia ka lyticase).
Nā huahana huahana
NO KA MEA E KOHO AI IA US
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