ʻO TIANprep Rapid N96 Plasmid Kit

Hana kiʻekiʻe, hemo wikiwiki ʻana o ka plasmid liʻiliʻi.

Lawe ka kit i ke ʻano hoʻoue isopropanol e loaʻa ai iā DNA plasmid. Hāʻawi ka kit i kahi papa helu kānana 96-maikaʻi N a me Buffer III, i hoʻomohala kūikawā ʻia e TIANGEN, a hiki ke kiʻi wikiwiki a me ka maikaʻi i ka DNA plasmid maʻemaʻe kiʻekiʻe. He kūpono ia no ka hoʻomākaukau wikiwiki ʻana o nā plasmids kiʻekiʻe-kiʻekiʻe. 1.3 ml o ka moʻomeheu koʻohune plasmid kope kiʻekiʻe hiki ke hoʻoponopono ʻia i kēlā me kēia luawai, a he 96 mau laʻana o ka plasmid DNA i huki wikiwiki ʻia. ʻO ka hua nui i kēlā me kēia luawai ʻo 10-20 μg (pili ka hua i nā kumu e like me ka helu kope plasmid, ka maʻi bacteria, a pēlā aku.). Hiki ke hoʻohana ʻia ka Plasmid DNA i lawe ʻia e ka kit i nā hoʻokolohua biology molekiola e like me ka digestion enzyme digestion, sequencing, library screening, ligation and transformation.

Pōpoki. Aʻole: Ka hōʻiliʻili nui
4992868 4 preps
4992869 24 preps

ʻIke kikoʻī

Kahua hana

Laʻana Hoʻokolohua

Nā huahana huahana

Hiʻona

■ Loaʻa a maʻalahi hoʻi: hiki ke lawe ʻia mai he 96 mau laʻana i 2-3 mau hola.
■ Hoʻokomo ʻia me kahi mea kikoʻī TIANRed kū hoʻokahi, hiki iā ia ke ʻike maopopo i ke kēkelē o ka lysis o ka hāpana i ka hana unuhi e hōʻoia i ka hoʻomaʻemaʻe kiʻekiʻe plasmid DNA.
■ Maʻemaʻe kiʻekiʻe: Hiki ke hoʻohana pololei ʻia ka plasmid maʻemaʻe kiʻekiʻe i nā hoʻokolohua kahe.

Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)


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    TIANprep Rapid N96 Plasmid Kit01

    TIANprep Rapid N96 Plasmid Kit02 Ua lawe ʻia ʻo Plasmid DNA mai ka 1 ml o ka poli Escherichia coli (TOP10) i hana ʻia i ka pō (LB medium) me ka hoʻohana ʻana iā TIANprep Rapid N96 Plasmid Kit, a me ka awelika o 8-10 μg o ka maʻemaʻe kiʻekiʻe pBS plasmid i loaʻa.
    2 µl plasmid i hoʻoili ʻia i loko o kēlā me kēia luawai o kahi gel agarose 1%, a ʻike ʻia e electrophoresis.
    TIANprep Rapid N96 Plasmid Kit03 Ua lawe ʻia ʻo Plasmid DNA mai ka 1 ml o ka poli Escherichia coli (TOP10) i hana ʻia i ka pō (LB medium) me ka hoʻohana ʻana iā TIANprep Rapid N96 Plasmid Kit, a me ka awelika o 8-10 μg o ka maʻemaʻe kiʻekiʻe pBS plasmid i loaʻa.
    Vector: pBS plasmid; Pākuʻi: TOP10.
    Hoʻopau ʻia ka plasmid e ABI. PRISM 3730XL DNA mea hoʻonoho
    Kākau i kāu leka ma aneʻi a hoʻouna iā mākou