■ ʻO ka maʻemaʻe kiʻekiʻe: Ua ʻae ʻia ka ʻenehana hoʻoiho endotoxin kū hoʻokahi e wehe pono i ka endotoxin.
RSS
■ Hoʻololi kūpono kiʻekiʻe: kūpono no ka transfection o ka nui o nā laina pūnaewele me nā pūnaewele endotoxin-sensitive.
■ Ka laulā o nā noi: hiki ke hoʻopili ʻia ka plasmid i hoʻomaʻemaʻe ʻia i ka transfection o nā holoholona a me nā mea kanu a me nā hoʻokolohua biology molekiola.
Hiki ke hoʻohana ʻia ka DNA plasmid i lawe ʻia me ka hoʻohana ʻana i kēia kini no nā hana like ʻole, e like me ka digestion enzyme digestion, PCR, sequencing, ligation, transformation and transfection of various cells.
Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)
Hiki i ka endotoxin wehe ʻokoʻa reagent Buffer ER ke hoʻopau pono i nā koena endotoxin i ka ʻōnaehana hopena, a hiki ke kiʻi ʻia i ka plasmid kiʻekiʻe. ʻO ke koena endotoxin plasmid he ≤ 0.1 EU / ml. | |
Hoʻomaʻemaʻe ka plasmid me ka hoʻohana ʻana iā EndoFree Maxi Plasmid Kit V2 a me nā huahana like mai ka mea hoʻolako 1 a me ka mea hoʻolako 2 i elute me ka nui o ka buffer elution. 1 μl plasmid i hoʻouka ʻia i kēlā me kēia laina e kuhi i ka nui o ka plasmid. 100 ng o ka plasmid nona ka manaʻo i hoʻoholo ʻia e ka spectrophotometer i hoʻouka ʻia i loko o ka gel hoʻokahi e hoʻoholo inā he kiʻekiʻe kiʻekiʻe ka waiwai o ke kukuna. Panina: Hōʻike ka hopena electrophoresis i ka hoʻohui maoli o ka plasmid i lawe ʻia e ka EndoFree Maxi Plasmid kit V2, inā he kope kiʻekiʻe a kope liʻiliʻi paha, ʻoi aku ke kiʻekiʻe ma mua o ka mea i lawe ʻia e nā mea hoʻolako 1 a me 2. |
Mai kona hoʻokumu ʻia ʻana, ke hoʻomohala nei kā mākou hale hana i nā huahana papa honua mua me ka hoʻopaʻa ʻana i ke ʻano
o ka maikaʻi ma mua. Ua loaʻa i kā mākou huahana ka maikaʻi maikaʻi loa i ka ʻoihana a me ka waiwaiabletrusty i waena o nā mea kūʻai aku a kahiko.