ʻO Reagent RNAstore

Mea hoʻouka hau ʻole no ka pale ʻana i ka pono o ka laʻana RNA.

He wai ka RNAstore Reagent, he mea mālama mālama kino ʻino ʻole. Komo koke ia i loko o nā hunaola a pale i nā hunaola kū ʻole mai RNA ma kahi e kaohi pono ʻana i ka hana RNase, e kūpono ana no ke kālailai ʻana i ka hōʻike ʻana o nā ʻola i nā ʻano pilikino.
No ka mālama ʻana i nā hāpana kiko, hiki ke hoʻokomo koke ʻia i nā kiko i ka RNAstore no ka mālama ʻana e hōʻalo i ka hoʻohaʻahaʻa o ka RNA, no laila ʻaʻole pono e hana koke ʻia ka hāpana a i ʻole ka hau i loko o ka wai nitrogen.
Hiki ke hoʻohana ākea ʻia ʻo RNAstore Reagent i nā laʻana like ʻole o ka vertebrate, e like me ka lolo, ka puʻuwai, ka puʻuwai, ka ʻāpā, ke ake, ka akemā a me ka thymus.

Pōpoki. Aʻole	Ka hōʻiliʻili nui
4992727	100 ml

E hoʻouna leka uila iā mākou

ʻIke kikoʻī

Laʻana Hoʻokolohua

Nā huahana huahana

Hiʻona

■ Nā kūlana mālama: hiki ke mālama ʻia kēia pahu hopu no 1 pule ma ka mahana o ka lumi, 1 lā ma 37 ℃, a ma ka liʻiliʻi he 1 mahina ma 4 ℃. No nā laʻana kiko, e hoʻohaʻahaʻa i ka 4 ℃ i ka pō hoʻokahi, a hoʻolilo i -20 ℃ a i ʻole -80 ℃ no ka mālama wā lōʻihi.
■ Hoʻokiʻi hou a hoʻoheheʻe hoʻi: hiki i ka paʻahau i ka hau -20 ℃ a i ʻole -80 ℃ ke hoʻokuʻu ʻia no 20 mau manawa me ka hoʻololi ʻole i ka maikaʻi o ka unuhi RNA.
■ Nā noi i lalo: Ma hope o ka hemo ʻana o ka hāpana mai ka RNAstore Reagent, hiki ke huki ʻia ka RNA āpau e TIANGEN's TRNzol, RNAprep Pure, RNAsimple reagents a me nā pahu.

Nā memo nui

■ Kūpono wale ʻo RNAstore no nā mea kiko manu hou.
■ ʻAʻole kūpono ʻo RNAstore i nā mea kanu mea kanu mea kanu.
■ Pono ka lakio helu o nā laʻana kiko a me RNAstore Reagent ma kahi o 1:10 (e laʻa no ka 100 mg kiko, ma ka liʻiliʻi he 1 ml o RNAstore e pono ai).
■ ʻO ka mānoanoa o kēlā me kēia ʻaoʻao o ka laʻana ʻaʻole pono e ʻoi aku i ka 0.5 cm e hōʻoia i ka hiki i ka RNAstore ke komo koke i loko o ka meaola.

Hiki ke hoʻonohonoho pono ʻia nā huahana āpau no ODM / OEM. No nā kikoʻī,e kaomi i ka lawelawe i hoʻopilikino ʻia (ODM / OEM)

Previous: ʻIke DNA Hi-Swab

Aʻe: Pahu RNAclean

Material: 15 mg ʻiʻo ake kolo iʻa
Nā hana: 0.5 g mau ʻiʻo ate iole (mālama ʻia ma RNAstore Reagent) i mālama ʻia ma 37 ℃, ka mahana o ka lumi a me 4 ℃ pākahi. Hoʻokaʻawale ʻia ka nui o ka RNA mai 15 mg ʻiole ʻiole ʻiole i mālama ʻia i nā mahana like ʻole me ka hoʻohana ʻana iā TRNzol Reagent (Cat. No. 4992730).
Nā hopena: E ʻoluʻolu e ʻike i ke kiʻi ʻelala agarose gel electrophoresis ma luna.
2-4 μl o 100 μl eluates i hoʻouka ʻia i kēlā me kēia laina.
C (kaohi maikaʻi): ua mālama pono ʻia nā hāpana kiko ma -80 ℃.
Ua alakaʻi ʻia ka electrophoresis ma 6 V / cm no 30 min ma 1% agarose.

Material: 15 mg ʻiʻo ake kolo iʻa
Nā hana: 0.5 g nā ʻiole ate o ka ʻiole (mālama ʻia ma RNAstore Reagent) i freezethawed no 5, 10, 15 a me 20 mau manawa pākahi. Ua hoʻokaʻawale ʻia ka nui o ka RNA mai 15 mg ʻiole ʻiʻo ʻiole iʻa no nā manawa like ʻole i hoʻohana ʻia me TRNzol Reagent (Cat. No. 4992730).
Nā hopena: E ʻoluʻolu e ʻike i ke kiʻi ʻelala agarose gel electrophoresis ma luna. 2-4 μl o 100 μl eluates i hoʻouka ʻia i kēlā me kēia laina.
C (kaohi maikaʻi): ua mālama pono ʻia nā hāpana kiko ma -80 ℃.
5, 10, 15, 20: nā manawa hoʻohālikelike-thawed o nā laʻana.
Ua alakaʻi ʻia ka electrophoresis ma 6 V / cm no 30 min ma 1% agarose.

Kākau i kāu leka ma aneʻi a hoʻouna iā mākou