ʻO ka ʻenehana diagnostic Molecular, keu hoʻi ka ʻike pathogen metagenomic (mNGS), he maikaʻi nā noi noi no ka ʻike pathogen kuʻuna, ʻike ʻole ʻia ka ʻike pathogen hou, ʻike maʻi maʻi ahupapa, ʻike kūpaʻa lāʻau, ʻike loiloi o ka hopena kanaka a me ka loiloi ʻana i ka hopena anti anti efficacy. Hāʻawi ia i kahi ala nui no ka pale a me ka kaohi ʻana o nā maʻi lele, a paipai i ka hoʻomaikaʻi ʻana o ka hiki diagnostical maʻi.
Hiki i ka mNGS ke kālailai holoʻokoʻa i ka microbial a me ka hoʻokipa ʻana i nā mea kūlohelohe (DNA a me RNA) i nā laʻana o nā mea maʻi, a hoʻololi mālie hoʻi mai ke keʻena hoʻokolohua a i ka noi lāʻau lapaʻau, ma ke ʻano he kumu no ka ʻike ʻokoʻa a nā kauka, a hāʻawi i ke kākoʻo no ke kuhi manaʻo.
Hāʻawi ʻo TIANGEN i ka hopena o ka unuhi microorganism kumu a me ka kūkulu hale waihona mNGS.
Ke pane nei i ka COVID-19 Pandemi
Mai ka wā o ka breakout COVID-19, ua hāʻawi ʻo TIANGEN i 5 mau miliona mau hōʻike e like me nā mea maka no ka wehe ʻana o ka virus nucleic acid a me nā mea ʻike pono fluorescent no nā mea ʻoi aku ma mua o 200 o nā mea hana loiloi hou a me nā hui ʻike ma ʻAsia, ʻAkau ʻAkau a me ʻAmelika Hema, a me nā ʻāina ʻEulopa. A ke kōkua nei i nā miliona o ka poʻe ma mua o 30 mau ʻāina a puni ka honua.
Ua ʻike ʻia nā huahana ʻoihana huakaʻi TIANGEN, e like me nā mea maka, i loko o ka hōʻike loiloi no ka hoʻohana pilikia ʻana o COVID-19 i hoʻokuʻu ʻia e ka World Health Organization i Iune 2020, i helu ʻia i loko o ka papa inoa i manaʻo ʻia o nā loiloi COVID-19 hou a puni ka honua i hoʻokuʻu ʻia e ka Kālā Global ma Ianuali 2021.
Mai nā mea maka e hoʻopau i nā huahana
kaohi paʻa ʻia nā loulou āpau ma lalo o ISO13485 ʻōnaehana hoʻokele maikaʻi
Iecaianoaaiiuo Aneie
Nā Mea Pono
Nā huahana semi-huahana
Hoʻokumu ʻia ʻo QC-NGS
Mālama Laʻana
Laʻana Pretreatment
Kaʻi ʻĀkika Nucleic
NGS
ʻO ka laʻana ʻōpala waha waha mālama
ʻO Reagent RNAstore
Palekana RNA i ka paʻahau
Noi: ka lolo, ka puʻuwai, ka puʻuwai, ka pelupelu, ka ate, ka māmā a me ka thymus, etc.
Mālama ʻo RNA: 1 lā ma 37 ° C, 7 mau lā ma 15-25 ° C, a i ʻole 4 mau pule ma 2-8 ° C. -20 ° C a i ʻole -80 ° C
Tissue hāpana wili
Pākuʻi Homogenization
Kūpono ia no ka unuhi ʻana o DNA / RNA / protein mai nā mea kanu / holoholona, nā lepo, nā feces, nā fungi, a pēlā aku.
Ka mahana hana: ma lalo o -10 ℃
Hana: 1-24 mau laʻana
Loaʻa ka hopena o ka RNA holoholona ma hope o ka wili ʻana e H24R
Ka nui o ka laʻana: 20 mg (puʻuwai: 10mg) Laʻana: Crucian carp Marker: DNA MarkerIII （TIANGEN ， MD103-02） RNA Extraction Kit: RNA Easy Fast Tissue / Cell Kit (TIANGEN, 4992732) Ka RNA i lawe ʻia ma hope o ka wili ʻana e TGrinder H24R is o ka noʻonoʻo kiʻekiʻe a me ka maʻemaʻe.
Hana Pūnaewele Nucleic Acid Extractor
● 32 a me 96-kaha i koho ʻia.
● Kuhi wikiwiki ʻana o ka huakiko nukelika maʻi ma loko o 30 min.
● Loaʻa nā kime reagent prefill kiʻekiʻe kiʻekiʻe no ka hana maikaʻi loa.
E wehe i kahi hoʻonā ʻoihana kiʻekiʻe
Mākēneki Hi-DNA / RNA Kit (4992408 -T4A)
● Hoʻohui kiʻekiʻe, kūlike kūpono me nā nukelika acid extractor maʻamau i ka mākeke.
● Hoʻonohonoho ʻia nā ʻōpala i hoʻopilikino ʻia a me nā lawelawe OEM e like me nā makemake kikoʻī.
● Kūlike: KingFisher, Hamilton, Beckman Coulter, Chemagen etc.
ʻO ka hāmeʻe PCR amplifi cation maoli o TIANGEN 4992408
ʻO ka hāmeʻe PCR amplifi cation maoli o ka mea hoʻolako T.
Ua hoʻoheheʻe ʻia ʻo AIV-H5 i 10-6 -10-8gradients me ka wai Milli-Q, a laila unuhi ʻia e King Fisher Flex. 200 μl o kēlā me kēia hāpana i noi ʻia. Ua hōʻike ʻo TIANGEN kit i ka ʻike maikaʻi a me ke kūpaʻa maikaʻi. Mea kani PCR manawa maoli: ABI 7500 Mea ʻike PCR manawa maoli Reagent: FP314
Pāʻālau ʻĀpana Manuahi
● Pono wale nā pono maʻalahi e hoʻopau i ka hoʻokolohua unuhi
● Hoʻomoʻa kaʻawale ʻia nā mea kūʻai aku i mea e pale ai i ka haumia o ke keʻa
● ʻO ka manawa pōkole pōkole a me ka hana maʻalahi, me ka pono kiʻekiʻe.
● Hoʻonohonoho ʻia nā ʻōpala i hoʻopilikino ʻia a me nā lawelawe OEM e like me nā makemake kikoʻī.
ʻO TIANamp Virus DNA / RNA Kit (4992285)
ʻO TIANamp Virus RNA Kit (4992286)
ʻO TIANamp Micro DNA Kit (4992287)
| ʻO Cat.no. | ʻAno acid nukelea | Ke ʻano hāpana pono |
| 4992285 | DNA / RNA | Serum, plasma, wai o ke kino, aʻaʻa, hopena hoʻōla swab, medium culture virus, a pēlā aku |
| 4992286 | RNA | Serum, plasma, wai o ke kino, aʻaʻa, hopena hoʻōla swab, medium culture virus, a pēlā aku |
| 4992287 | DNA | ʻO ke koʻohune, fungi, parasites a me nā mea ʻino i hoʻokaʻawale ʻia mai ke serum, plasma, nāʻaʻaʻa, nā pleura a me nā ascites, ka cerebrospinal fluid, sputum, bronchoalveolar lavage fluid a me paraffin section |
● TIANSeq DirectFast Library Kit (illumina) (4992259/4992260)
ʻO ka ʻāpana DNA Enzymatic. He kūpono no ke kūkulu hale waihona DNA no Illumina kiʻekiʻe ma o ka paepae hoʻokalakupua
● TIANSeq Fast DNA Library Kit (illumina) (4992261/4992262)
He kūpono no ke kūkulu hale waihona DNA no Illumina kiʻekiʻe ma o ka paepae hoʻokalakupua
● Pahu Waihona Pūnaewele DNA wikiwiki no ka paepae Ion Torrent (kit i hoʻonohonoho ʻia)
Kūpono no ke kūkulu hale waihona DNA no ke kahawai kiʻekiʻe ʻo Ion torrent
● Pahu Waihona DNA wikiwiki no ka papaha MGI (kit i hoʻonohonoho ʻia)
Kūpono no ke kūkulu hale waihona DNA no MGI kiʻekiʻe ma o ka paepae hoʻokaʻawale pūnaewele
● TIANSeq rRNA Depletion Kit (H / M / R) (4992363/4992364/4992391) (no ke kahawai Illumina / Ion / MGI)
No ka hemo ʻana o ka host rRNA i mea e hoʻomaikaʻi ai i ka ʻike o ka ʻike RNA viral
● ʻO TIANSeq Fast RNA Library Prep Kit (Illumina) (4992375)
No ke kūkulu ʻana i ka waihona RNA virus ma hope o ka hemo ʻana o ka host RRNA, kahi e hiki ai ke hoʻoholo koke i ke ʻano o ke kaʻina RNA virus
● ʻO TIANSeq Straded RNA-Seq Kit (Illumina) (4993007)
No ke kūkulu ʻana i ka waihona RNA virus ma hope o ka hemo ʻana o ka host RRNA, kahi e hiki ai ke hoʻoholo pono i ke kaʻina ʻokoʻa o ka viral
● Pūnaewele Hoʻomākaukau RNA RNA Library no ka Mīkini MGI (kit i hoʻonohonoho ʻia)
Kūpono no ke kūkulu hale waihona puke RNA no ka papahumehana hoʻoliʻiliʻi kiʻekiʻe kiʻekiʻe o MGI
● TIANSeq Size Selection DNA Beads (4992358/4992359/4992979)
No ka hoʻomaʻemaʻe a me ka nui o ke koho ʻana o nā ʻāpana DNA i ka wā o ka hale waihona puke DNA
● ʻO TIANSeq RNA Nā Pulu Maʻemaʻe (4992360/4992362/4992867)
No ka hoʻomaʻemaʻe RNA ma hope o ka hoʻonui ʻana o RNA
● TIANSeq Single-Index Adapter (Illumina) (4992642/4992378)
● TIANSeq Dual-Index Adapter (Illumina) (NG216-T1 / 2/3/4/5/6)
No ke kūkulu hale waihona DNA no Illumina kiʻekiʻe ma o ka paepae hoʻokaʻawale pūnaewele
